Acid Fast Stain (Kinyoun-Cold Method)
Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic …
Staining
Acridine Orange Stain- Principle, Procedure and Result Interpretation
Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical …
Read moreAcridine Orange Stain- Principle, Procedure and Result Interpretation
Simple Staining- Principle, Procedure and Result Interpretation
True to its name, the simple stain is a very simple staining procedure involving only one stain. You may choose from methylene blue, Gram safranin, and Gram crystal violet. Basic stains, …
Read moreSimple Staining- Principle, Procedure and Result Interpretation
Negative Staining- Principle, Procedure and Result Interpretation
Objectives of Negative Staining To perform a negative staining procedure. To understand the benefit obtained from visualizing unstained microorganisms. Principle of Negative Staining Negative staining …
Read moreNegative Staining- Principle, Procedure and Result Interpretation
Capsule Stain- Principle, Procedure and Result Interpretation
A capsule is a gelatinous outer layer that is secreted by the cell and that surrounds and adheres to the cell wall. It is not …
Read moreCapsule Stain- Principle, Procedure and Result Interpretation
Spore Stain- Principle, Procedure and Result Interpretation
Members of the anaerobic genera Clostridium and Desulfotomaculum and the aerobic genus Bacillus are examples of organisms that have the capacity to exist either as metabolically active vegetative cells or as …
Read moreSpore Stain- Principle, Procedure and Result Interpretation