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Staining

Acid Fast Stain (Kinyoun-Cold Method)

by Amit Sabhadiya

Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic …

Read moreAcid Fast Stain (Kinyoun-Cold Method)

Categories Staining

Acridine Orange Stain- Principle, Procedure and Result Interpretation

by Amit Sabhadiya

Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical …

Read moreAcridine Orange Stain- Principle, Procedure and Result Interpretation

Categories Staining

Simple Staining- Principle, Procedure and Result Interpretation

by Amit Sabhadiya

True to its name, the simple stain is a very simple staining procedure involving only one stain. You may choose from methylene blue, Gram safranin, and Gram crystal violet. Basic stains, …

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Categories Staining

Negative Staining- Principle, Procedure and Result Interpretation

by Amit Sabhadiya

Objectives of Negative Staining To perform a negative staining procedure. To understand the benefit obtained from visualizing unstained microorganisms. Principle of Negative Staining Negative staining …

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Categories Staining

Capsule Stain- Principle, Procedure and Result Interpretation

by Amit Sabhadiya

A capsule is a gelatinous outer layer that is secreted by the cell and that surrounds and adheres to the cell wall. It is not …

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Categories Staining

Spore Stain- Principle, Procedure and Result Interpretation

by Amit Sabhadiya

Members of the anaerobic genera Clostridium and Desulfotomaculum and the aerobic genus Bacillus are examples of organisms that have the capacity to exist either as metabolically active vegetative cells or as …

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Categories Staining

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