How To Operate A Microscope


How To Operate A Microscope?

Part of a video titled BIOLOGY 10 - Basic Microscope Setup and Use - YouTube



Lens. Start with a low-power objective lens 4x. And the stage in its highest position. Then lookMoreLens. Start with a low-power objective lens 4x. And the stage in its highest position. Then look through the ocular lens. And focus on the specimen.

How do you operate a microscope step by step?

Steps on How to Use a Light Microscope
  1. Step 1: Connect the light microscope to a power source. …
  2. Step 2: Turn the revolving nosepiece so the lowest objective lens is in position.
  3. Step 3: Mount your specimen onto the stage. …
  4. Step 4: Use the metal clips to keep your slide in place.

How do you safely operate a microscope?

Your microscope slide should be prepared with a coverslip over the sample to protect the objective lenses if they touch the slide.
  1. Do not touch the glass part of the lenses with your fingers. …
  2. Always keep your microscope covered when not in use.
  3. Always carry a microscope with both hands.

What are the 10 steps in using microscope?

How To Use Your First Microscope
  1. Step 1: Moving Your Microscope. Carry the microscope with two hands. …
  2. Step 2: Microscope Lens Care. Never touch any lens with your fingers. …
  3. Step 3: Microscope Parts. …
  4. Step 4: Prepare a Slide. …
  5. Step 5: Insert the Slide. …
  6. Step 6: Set Up for Viewing. …
  7. Step 7: Light Control. …
  8. Step 8: Focus the Microscope.

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How do you focus a microscope step by step?

What are the 3 main parts of microscope?

The three basic structural components of a compound microscope are the head base and arm.
  • Head/Body houses the optical parts in the upper part of the microscope.
  • Base of the microscope supports the microscope and houses the illuminator.
  • Arm connects to the base and supports the microscope head.

What is the formula for magnification?

The magnification equation states that M = Hi/Ho = – Di/Do where M is the magnification Hi is the height of the image Ho is the height of the object Di is the distance from the lens to the image and Do is the distance of the object to the lens.

What are 4 precautions one should take when handling a microscope?

Safety Tips With Microscopes
  1. Wear Protective Clothing. Before you begin using a microscope put on protective clothing. …
  2. Carry with Two Hands. …
  3. Do Not Touch the Lens. …
  4. Do Not Look into the Light. …
  5. Be Cautious Handling Slides. …
  6. Storing.

What are the proper precautions and handling of the microscope?

Microscope Safety
  • Clean the microscope after each use. …
  • Handle glass slides carefully. …
  • Turn off the light source when the microscope is not in use. …
  • Be aware if your microscope has a mercury lamp. …
  • When carrying the microscope always use two hands with one hand supporting the base and theother hand holding the arm.

How do you handle and take care of a microscope?

Proper care and maintenance of your microscope can extend its life by many years.
  1. Tip 1: Handle with care. …
  2. Tip 2: Keep lenses clear of slides. …
  3. Tip3: Clean after using immersion oil. …
  4. Tip 4: Cover when not in use. …
  5. Tip 5: Look after the bulb. …
  6. Tip 6: Store in a clean dry place.

How do you use a microscope to view a slide?

How to use a microscope
  1. Move the stage (the flat ledge the slide sits on) down to its lowest position.
  2. Place the glass slide onto the stage. …
  3. Select the lowest power objective lens.
  4. Turn the coarse focus knob slowly until you are able to see the cells.

How do you adjust a microscope?

Adjusting a Microscope
  1. Turn on the lamp and adjust its intensity.
  2. Adjust the interpupilary distance so that the two circles of light merge into one.
  3. Place a sample on the stage.
  4. Select the 10X objective and look through the right eyepiece only.

What does the stage clips do on a microscope?

Stage clips hold the slides in place. If your microscope has a mechanical stage you will be able to move the slide around by turning two knobs. One moves it left and right the other moves it up and down.

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How do you focus a slide?

Viewing Prepared Slides

Start by rotating the objective lens to lowest power. Place a slide on the stage label side up with the coverslip centered. On LOW POWER ONLY use the coarse focus knob to get the object into focus. If you cannot see anything move the slide slightly while viewing and focusing.

How do you adjust the slide on a microscope?

Slowly move the slide to center the specimen under the lens if necessary. Do this by nudging it gently with your fingers or by turning the slide control knobs if you have a mechanical stage. Adjust the small fine focus knob until the specimen is clearly in focus. Then adjust the diaphragm to get the best lighting.

What are the 5 steps in focusing on a microscope?

Terms in this set (11)
  1. Plug in the microscope.
  2. Turn the microscope light on.
  3. Place the stage as far away from the objective lenses as possible.
  4. Rotate the revolving nosepiece until the lowest power objective lens clicks into place.
  5. Looking at your stage from the side place the specimen over the hole in the stage.

What holds the slide in place on a microscope?

Use the stage clips to hold the slide in place.

What does a diaphragm do on a microscope?

Opening and closing of the condenser aperture diaphragm controls the angle of the light cone reaching the specimen. The setting of the condenser’s aperture diaphragm along with the aperture of the objective determines the realized numerical aperture of the microscope system.

What does a condenser do on a microscope?

On upright microscopes the condenser is located beneath the stage and serves to gather wavefronts from the microscope light source and concentrate them into a cone of light that illuminates the specimen with uniform intensity over the entire viewfield.

How do you calculate a bar scale?

Scale bar
  1. Measure the scale bar image (beside drawing) in mm.
  2. Convert to µm (multiply by 1000).
  3. Magnification = scale bar image divided by actual scale bar length (written on the scale bar).

WHAT IS lens power?

The power of a lens is defined as the reciprocal of the focal length. Lens power is measured in dioptres (D). … Diverging (concave ) lenses have negative focal lengths so they also have negative power values.

How do you measure the size of a cell under a microscope?

Divide the number of cells in view with the diameter of the field of view to figure the estimated length of the cell. If the number of cells is 50 and the diameter you are observing is 5 millimeters in length then one cell is 0.1 millimeter long. Measured in microns the cell would be 1 000 microns in length.

What techniques do you use to properly place a sample in a microscope?

The main methods of placing samples onto microscope slides are wet mount dry mount smear squash and staining. Dry Mount: The dry mount is the most basic technique: simply position a thinly sliced section on the center of the slide and place a cover slip over the sample.

What are the don’ts in using a microscope?

The Don’ts:
  • a. Never touch a lens with anything but lens paper.
  • b. Never touch a lens with dry lens paper.
  • c. Never rub a lens.
  • d. Never put oil on a dry objective and remove it at once if an accident occurs.
  • e. Never wear mascara when using a microscope.

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What are the do’s and don’ts in using a microscope?

Don’t touch the lenses of the microscope with your fingers. Keep slides and cover slips clean. Use anything OTHER than lens paper to clean them. NEVER aim the Lumarod or microscope at the sun or look directly into the sun.

How do you prepare a specimen slide?

How do cheek cells look under a microscope?

Without stains cells would appear to be almost transparent making it difficult to differentiate its parts. … The nucleus at the central part of the cheek cell contains DNA. When a drop of methylene blue is introduced the nucleus is stained which makes it stand out and be clearly seen under the microscope.

Can you see cells under a microscope?

Microscopes provide magnification that allows people to see individual cells and single-celled organisms such as bacteria and other microorganisms. Types of cells that can be viewed under a basic compound microscope include cork cells plant cells and even human cells scraped from the inside of the cheek.

How is magnification adjusted?

The magnification adjusts through combining the eyepiece and lenses of most microscopes. … Calculate total magnification by multiplying the eyepiece magnification by the objective lens magnification. Typical laboratory microscopes magnify objects 40x 100x and 400x.

What do you adjust first on a microscope?


What does the coarse focus knob do on a microscope?

COARSE ADJUSTMENT KNOB — A rapid control which allows for quick focusing by moving the objective lens or stage up and down. It is used for initial focusing.

What is the HPO in microscope?

HPO stands for High Power Objective (microscope lens)

How do we center the specimen?

1. Place the microscope slide with the specimen within the stage clips on the fixed stage. Move the slide to center the specimen over the opening in the stage directly over the light source.

What is the iris diaphragm on a microscope?

Medical Definition of iris diaphragm

: an adjustable diaphragm of thin opaque plates that can be turned by a ring so as to change the diameter of a central opening usually to regulate the aperture of a lens (as in a microscope)

BIOLOGY 10 – Basic Microscope Setup and Use

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How to Use a Microscope | STEM

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