What Factors Besides Physical Growth Characteristics Of The Organism Are Important?
What factors besides physical growth characteristics are important when recording data about an organism? Other important factors to include would be the growth medium incubation temperature and atmosphere (aerobic or anaerobic).
What other information should you include when describing physical growth characteristics?
What other information should you include when describing the physical growth characteristics? Incubation temperature time and the type of medium used—these all affect the appearance of bacterial growth. Colony size color and shape are three critical aspects of a description of bacterial growth.
Why are growth characteristics more useful on agar plates than agar slants?
Why are growth characteristics more useful on agar plates than on agar slants? … Slants are better suited because they can be capped preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
Which type of culture media is best used to grow stock cultures of bacteria for storage quizlet?
Agar is an ideal solidifying agent for microbial growth media because it is indigestible which means that the microbes do not utilize it as a nutrient source they only utilize the specific nutrients that are in the media. Also agar remains solid at the temperatures that are often used to grow microbes 37oC and 25oC.
Which of the following term describes growth as opaque dense with smooth edge?
Filiform– dense and opaque with a smooth edge. pellicle- some organisms float on top of the medium and produce a type of surface membrane called a pellicle.
Why is it important to characterize and identify unknown environmental bacteria?
WHY IS THE IDENTIFICATION OF BACTERIAL UNKNOWNS IMPORTANT? Microbiologists must identify bacterial isolates for several practical reasons: • Medical diagnostics — identifying a pathogen isolated from a patient. Food industry — identifying a microbial contaminant responsible for food spoilage.
What is the importance of describing the cultural colonial characteristics of bacteria?
What are some advantages of an agar plate over a slant tube?
What are some advantages of an agar plate over a slant tube? Agar slants are useful in maintaining growth of microorganism over long periods of time without allowing the cultures to dry out. The agar plate is beneficial over a slant tube because it allows for observation easy access and isolation of various cultures.
Why is slant culture preferred in preservation over Petriplate culture?
Slanting the surface of the agar gives the bacteria a greater surface area on which to grow in a test tube. Furthermore slants are created in test tubes that can be capped which minimizes water loss.
What are slant cultures in microbiology quizlet?
Broth cultures provide a large amounts of bacteria in small volume and they are easily transported. Describe agar slants. Agar slants are test tubes containing solid media that were left an an angle while the agar solidified. They provide a solid growth surface for bacteria and are also easily stored and transported.
What factors can greatly impact the growth of bacteria in an environment or on a particular object?
Warmth moisture pH levels and oxygen levels are the four big physical and chemical factors affecting microbial growth.
Why wouldn’t it be advisable to compare growth of the organisms on each plate to each other?
why wouldn’t it be advisable to compare growth of the organisms on each plate to each other? … So you can’t compare growth on selective media. Even on TSA the growth of different organisms is likely different.
What is the main benefit of using agar as a growth medium?
The main advantage of this agar is the absence of inhibitors which could interfere in the micro-organisms growth. It has excellent transparency high hysteresis and very reliable reproducibility.
What are the environmental factors that affect the growth of bacteria?
- Moisture. Water is an essential component for the growth of the bacteria. …
- Oxygen. On the basis of requirement of oxygen bacteria are classified under three groups. …
- Carbon Dioxide. …
- Temperature. …
- pH. …
- Light. …
- Osmotic Effect. …
- Mechanical and Sonic Stress.
Which of the following characteristics may be used to describe the growth of bacteria on a solid surface like an agar slant?
The term Colony Forming Unit (CFU) is used to describe bacterial growth on solid agar because bacterial cells stick together.
How are bacteria identified basing from gross colonial morphology?
Why is it important to identify and characterize bacteria?
In microbial ecology the identification of microorganisms helps us characterize biodiversity. … Because the clinical samples will most likely contain many microorganisms both normal flora and pathogens it is important to isolate the pathogen in a pure culture using various types of selective and differential media.
Which two characteristics are the most important to identify when starting to identify an unknown bacteria?
Begin the process of identifying unknown bacteria by observing their physical characteristics such as cell wall shape and linkages. Use standard laboratory procedures like cell staining culturing and DNA sequencing to further narrow down your identification.
Why is it important to identify an organism?
As highlighted in the Introduction the accurate identification of organisms is important when measuring the biodiversity of an area. … And without accurate identification it is impossible to determine how many species exist in a given area. Identifying organisms is however a task fraught with difficulties.
What is cultural characteristics of bacteria?
Abstract. The cultural characteristics of microbial colonies are analyzed on different kinds of media. … The following three characteristics are readily appearing from a single bacterial colony or more dense growth without the aid of any type of magnifying device: texture transparency and color or pigmentation.
What factors influence the colony morphology of a microbial growth quizlet?
Incubation temperature time and the type of medium used—these all affect the appearance of bacterial growth. Three critical aspects of a description of bacterial growth are colony size color and shape.
How are bacterial colonies characterized based on their appearance on the culture media?
Bacterial colonies are frequently shiny and smooth in appearance. Other surface descriptions might be: veined rough dull wrinkled (or shriveled) glistening.
How might you prepare a spread plate from organisms growing on a slant?
Inoculate the slant by transferring cells with an inoculating loop from a single-colony microorganism on a plate to the slant’s surface. Move the loop across the surface of the slant and recap the tubes. Incubate the slant until there is evidence of growth then put the tube in a refrigerator.
What is the purpose of using agar plate agar slant and broth in culture for microorganisms?
Agar plates will be used for isolation and some assays and for short term maintenance of cultures. Agar slant tubes will be used for long term maintenance of isolates. Broths (liquid media) will be used to grow isolates for some assays or for the assays themselves.
What are the advantages and disadvantages of spread plate method?
- The optimum method for aerobes while microaerophilic tends to grow slower.
- The dilutions should be Accurate.
- The volume of inoculum greater than 0.1 mL on the nutrient agar not soak well and may result in colonies to coalesce as they form.
Why is it important to have a pure culture and not a mixed culture when performing biochemical tests?
The importance of having a pure culture and not a mixed culture when performing biochemical testing is that a pure culture may react much differently in isolation than when it is combined with other species. … A mixed species entails contamination and as such the control of the experiment is lost.
Why is it important to keep the lid on your growth plates as much as possible?
keep the lid over the Petri dish as much as possible to prevent air contamination. This is a dilution technique bacteria are being deposited onto the agar surface as the loop moves across the plate.
Why is it important to preserve microorganisms?
Why are growth characteristics more useful on agar plates than agar slants?
Why are growth characteristics more useful on agar plates than on agar slants? … Slants are better suited because they can be capped preventing the agar and culture from drying out. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
Why would you grow organisms in broth media quizlet?
The broth culture allows a lot more bacteria to grow within a smaller area while there is little room for bacteria to grow on a slant. … Your microbiology lab maintains reference bacterial cultures which are regularly transferred to new nutrient agar slants.
What is the term for the plates slants or broths used to grow bacteria?
Broth- Slants- Plates or petri dishes– Nutrients in broth/agar used to culture microorganisms(bacteria)
What are the 4 main growth requirements for bacteria?
There are four things that can impact the growth of bacteria. These are: temperatures moisture oxygen and a particular pH.
What are the two most important factors to control the growth of bacteria?
- Food: Bacteria require food to survive. …
- Acid: Bacteria do not grow in acidic environments. …
- Temperature: Most bacteria will grow rapidly between 4°C and 60°C (40°F and 140°F). …
- Time: Bacteria require time to multiply. …
- Oxygen: There are two types of bacteria.
What causes the growth of bacteria?
Moisture – Bacteria need moisture in order to grow. … Food – Food provides energy and nutrients for bacteria to grow. High risk foods particularly protein foods such as chicken and dairy products are rich in nutrients and moisture and so promote bacterial growth.
Characteristics of Life
Microbiology – Bacteria Growth Reproduction Classification
Characteristics Of Living Organisms IGCSE Biology
FACTORS EFFECTING MICROBIAL GROWTH